c-Kit Y703F/Y936F Stable Ba/F3 Cell Line

CAT.NOUNITPRICE
T30481x106 cells / 1.0 ml
$0.00

Specifications


Descriptionc-Kit or stem cell factor (SCF) receptor is a type III tyrosine kinase receptor that has a major role in the hematopoietic system and has nine key tyrosine residues. Y703 and Y936 residues of the c-Kit receptor are involved in recruiting signaling molecules and are ligand-induced phosphorylation sites for Grb2. c-Kit Y703F/Y936F-expressing Ba/F3 cells (with GNNK- isoform) has a Y-to-F mutation at position 703 and 936, which results in reduced binding for Grb2. The GNNK- isoform is the outcome of the c-Kit gene alternative splicing sites, resulting in different isoforms of the tetrapeptide GNNK, and is the main transcript found in human malignancies. Together with the wild-type c-Kit stable Ba/F3 cells (Cat. No. T3041 and T3042), these cells may be utilized in studies involving effects of tyrosine residues on signaling pathways.
SKUT3048
SpeciesMouse (M. musculus)
Species descriptionMus musculus
Tissue/Organ/Organ SystemBone Marrow
Growth PropertiesSuspension
Cell MorphologyRound
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetKinases
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation RequirementsThe base medium for this cell line is Prigrow II medium available at abm, Cat. No. TM002. To make the completed growth medium, add the following components to the base medium: 10 ng/ml recombinant murine IL-3, fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QC1) Western blotting for presence of c-Kit; 2) PCR
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorLund University
Documents


Supporting Protocol
MSDS

    QC

      Other

      FAQs


      What is the biosafety level for these cells?
      In accordance to the U.S. Public Health Service Guidelines, the designation of Ba/F3, a murine Pro B cell line, falls under laboratory biosafety level 1 (BSL-1). Biosafety Level 1 is suitable for work involving well-characterized agents not known to cause disease in humans and present minimal potential hazard to the laboratory personnel and the environment. It is the responsibility of the end user to ensure that their facility comply with biosafety regulations for their own country. The regulation guidelines include, but not limited to: Enforcement of standard laboratory practices:
      • No eating, drinking and smoking permitted in laboratory areas
      • No mouth pipetting
      • Safe handling of sharps, such as needles, pipettes, broken glassware
      • Decontaminate cultures and waste in accordance to applicable regulations
      Use of Personal Protective Equipment (PPE):
      • Laboratory coats and gloves; eye, face protection as needed
      References


      There are no references for this product yet!