Cas9 Expressing Doxycycline Inducible HeLa (cTT20) Cell Line

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T61751x106 cells / 1.0 ml
$0.00

Specifications


DescriptionThis HeLa cell line expresses Cas9 when induced by doxycycline. A PiggyBac transposon system was used to introduce Cas9. This parental cell line is applicable for work requiring human CRISPR/Cas9 knockout cells. Lack of pre-existing guide RNA enables freedom to introduce custom gRNA for desired gene targets.
SKUT6175
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemCervix
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Seeding Density40,000 - 50,000 cells/cm2
Population Doubling Time24 - 34 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileNeomycin resistance
Preservation Protocol1. Freeze Medium: Complete growth medium with 50% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Western blot; 2) Immunofluorescence
Documents


Supporting Protocol
MSDS

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