DNA Purification Magnetic Beads (5 ml)
| Cat. No. | G950 |
| Name | DNA Purification Magnetic Beads (5 ml) |
| Unit | 4 x 1.25 ml |
| Description |
abm’s DNA Purification Magnetic Beads are carboxyl-coated paramagnetic particles that can reversibily bind to DNA. The magnetic beads offer flexibility as they can be used to size-select for DNA fragments and can be used to remove unwanted excess primers, adapter dimers, salts and enzymes from a wide variety of different reactions. Key Features:
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| Application |
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| Storage Condition |
Store tightly sealed at 4°C. Freezing may reduce binding efficiency of beads. |
| Note |
Bring to room temperature 30 minutes before use and vortex mix vigorously to thoroughly resuspend the beads. A magnetic separation rack is required (not included). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G950 |
| Can the DNA or RNA Purification Magnetic Beads be reused? | |
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No, the DNA or RNA Purification Magnetic Beads are intended for single-use only, particularly for RNA work where the contamination risk is high.
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| What are the advantages of using paramagnetic beads over silica-based spin columns for nucleic acid purification and concentration? | |
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Advantages include: automation compatible, scalable, flexible sample volumes, size selection capability, no centrifugation equipment required and superior reproducibility.
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| How do abm's DNA and RNA Purification Magnetic Beads work? | |
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Nucleic acids bind to the magnetic beads in the presence of a crowding agent and optimized buffer. Using a magnetic rack, the nucleic acid-bound beads are captured, washed to remove contaminants, and then the purified nucleic acids are eluted under aqueous conditions.
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| Are abm's DNA and RNA Purification Magnetic Beads compatible with automation? | |
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Yes. The provided Datasheets outline a manual workflow, however they are amendable to high-throughput and automated workflows.
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| My yield is lower than expected, what can I do to improve sample recovery when using abm's DNA or RNA Purification Magnetic Beads? | |
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1. Prior to use, ensure beads have come to room temperature (20-25°C) for at least 30 min, then vortex thoroughly until the solution is fully homogenous. 2. Ensure the correct binding ratio is utilized. 3. Check sample integrity prior to purification. 4. Use freshly prepared 70% or 80% ethanol (according to product Datasheet). 5. During the elution step, ensure beads are thoroughly resuspended and incubated with water or elution buffer. |
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