DyLight 488 Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]

SD002100 µg


DescriptionMinimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins
Unit quantity100 µg
Species ReactivityOther
ImmunogenRabbit IgG, whole molecule
ApplicationsThis antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting. This antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Recommended DilutionsFLUORESCENT WESTERN BLOT >1:20,000
ELISA >1:10,000
Concentration1.0 mg/ml (by UV absorbance at 280 nm)
Storage ConditionThis product is stable for several weeks at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. Expiration date is one (1) year from date of receipt if properly stored.
Storage Buffer0.01% (w/v) Sodium Azide
Emission Wavelength518 nm (in PBS)
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
ConjugationN-hydroxysuccinimide (NHS) ester
Stabilizer10 mg/ml BSA, IgG and Protease free
PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of rabbit IgG and with light chains of most rabbit immunoglobulins.
CautionThis product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin from Applied Biological Materials Inc. are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. DyLightâ„¢ is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

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