GFP Stably Expressing MCF7 Cell Line

T39041x106 cells / 1.0 ml


DescriptionMCF7 cells stably expressing GFP
SpeciesHuman (H. sapiens)
Species descriptionHuman
Tissue/Organ/Organ SystemBreast
Growth PropertiesAdherent
Growth PropertiesAdherent
Cell MorphologyEpithelial-Like
Population Doubling Time24-36 hours
Unit quantity1x106 cells / 1.0 ml
Propagation RequirementsThe base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the completed growth medium, add the following components to the base medium with the final concentration: fetal bovine serum (TM999) to a final concentration of 10%, and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QCPuromycin drug selection
CautionFor Research Use Only
Cell TypeReporter Cells
Expression ProfilePuromycin
Shipping ConditionsDry Ice

Supporting Protocol




        Is the GFP or RFP signal localized within the cell?
        No, there is no localization tag on the GFP or RFP, so they can diffuse throughout the cell. However, if you want a localized signal, abm's Organelle Labeling Lentiviruses can be used for our Stable Cell Line Generation service. Simply ask [email protected] for a quote.
        What type of GFP or RFP do these cell lines express, and from which promoter?
        The GFP is derived from copGFP (from Pontellina plumata) and the RFP is derived from mKate2 (from Entacmaea quadricolor). They are expressed from a proximal SV40 promoter, and a more distal CMV promoter may also drive expression. If you are looking for a cell line with a specific fluorescent reporter, or with a specific promoter, abm can perform a Stable Cell Line Generation service. Simply ask [email protected] for a quote.

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