Anti-His Tag Antibody (Mouse Monoclonal)

Cat. No.
100 μg
Cat. No. G020
Name Anti-His Tag Antibody (Mouse Monoclonal)
Unit 100 μg
Clone HIS.H8
Category Tag Antibodies
Application WB,IHC,IP,ICC/IF
Raised In Mouse
Clonality Tag
Reactivity Other

The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Immunogen 6x His synthetic peptide.
Isotype IgG2b
Concentration 100 µg/100 µl
Storage Buffer PBS, pH 7.4 with 0.05% sodium azide.
Storage Condition

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Formulation Liquid
Specificity His-tag fusion protein
Purity IgG fraction

abm guarantees that all our Anti-His Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G020
Print & Download Datasheet
  • Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate" Dev Biol 365 (2):353-75 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
  • Lim, KH et al. "Biotin-assisted folding of streptavidin on the yeast surface" Biotechnol prog 28 (1):276-283 (2012). DOI: 10.1002/btpr.721. PubMed: 21990063. Application: Immunoprecipitation.
  • Bae, HJ et al. "DBC1 does not function as a negative regulator of SIRT1 in liver cancer" Oncol Lett 4 (5):873-877 (2012). PubMed: 23162614. Application: Western Blot.
  • Liu, X et al. "Molecular and structural characterization of the SH3 domain of AHI‐1 in regulation of cellular resistance of BCR‐ABL+ chronic myeloid leukemia cells to tyrosine" Proteomics 12 (13:2094-2106 (2012). DOI: 10.1002/pmic.201100553. Application: Western Blot.
  • Park, UH et al. "Piperine, a Component of Black Pepper, Inhibits Adipogenesis by Antagonizing PPARγ Activity in 3T3-L1 Cells" J Agric Food Chem 60 (15):3853-3860 (2012). DOI: 10.1021/jf204514a. PubMed: 22463744. Application: Western Blot.
  • Mani, T et al. "FERM domain phosphoinositide binding targets merlin to the membrane and is essential for its growth-suppressive function" Mol Cell Biol 31 (10):183-1996 (2011). DOI: 10.1128/MCB.00609-10. Application: Immunoblot.
  • Neilson, KM et al. "Specific domains of FoxD4/5 activate and repress neural transcription factor genes to control the progression of immature neural ectoderm to differentiating neural plate" Dev Biol. 365:353-75 (2012). DOI: 10.1016/j.ydbio.2012.03.004. Application: Western Blot.
  • Paluzzi, JP et al. "The heterodimeric glycoprotein hormone, GPA2, GPB5, regulates ion transport across the hindgut of the adult mosquito, Aedes aegypti" PLoS ONE 9:e86386 (2014). PubMed: 24466069. Application: Western Blot.
  • Metzler, M et al. "NMDA receptor function and NMDA receptor-dependent phosphorylation of huntingtin is altered by the endocytic protein HIP1" J Neurosci 27:2298-308 (2007). PubMed: 17329427.
  • Tsai, SL et al. "Functional display of complex cellulosomes on the yeast surface via adaptive assembly" ACS Synth Biol 2:14-21 (2013). PubMed: 23656322.
  • Olmstead, AD et al. "Human subtilase SKI-1, S1P is a master regulator of the HCV Lifecycle and a potential host cell target for developing indirect-acting antiviral agents" PLoS Pathog. 8:e1002468 (2012). PubMed: 22241994.
  • Xue, Y et al. "FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair" Hum. Mol. Genet. 17:1641-52 (2008). PubMed: 18285517.
  • Tayapiwatana, C et al. "Novel 3′-Processing Integrase Activity Assay by Real-Time PCR for Screening and Identification of HIV-1 Integrase Inhibitors" BioMed Research International : (2015). DOI:
  • Choi, J. W. et al. "Recombinant Human Laforin Expressed in Insect Cells: Expression, Purification, and Biochemical Characterizations" J. Korean Chem. Soc. 5:466-470 (2015). DOI: 10.5012/jkcs.2015.59.5.466. Application: WB.
  • Kwon, Y et al. "Biomimetic repeat protein derived from Xenopus tropicalis for fibrous scaffold fabrication" Biopolymers. 12:659-664 (2015). DOI: 10.1002/bip.22735. PubMed: 26297878.
  • Belo, Y., Mielko, Z., Nudelman, H., Afek, A., Ben-David, O., Shahar, A., … Arbely, E. "Unexpected implications of STAT3 acetylation revealed by genetic encoding of acetyl-lysine" Biochimica Et Biophysica Acta (BBA) - General Subjects 1863(9):1343–1350 (2019). DOI: 10.1016/j.bbagen.2019.05.019.
  • Harada, H., Farhani, N., Wang, X.-F., Sugita, S., Charish, J., Attisano, L., … Monnier, P. P. "Extracellular phosphorylation drives the formation of neuronal circuitry" Nature Chemical Biology 15(11):1035–1042 (2019). DOI: 10.1038/s41589-019-0345-z.
  • Ko, K. Y., Lee, J. H., Jang, J. K., Jin, Y., Kang, H., & Kim, I. Y. "S-Glutathionylation of mouse selenoprotein W prevents oxidative stress-induced cell death by blocking the formation of an intramolecular disulfide bond" Free Radical Biology and Medicine 141:362–371 (2019). DOI: 10.1016/j.freeradbiomed.2019.07.007.
  • Min, H. J., Cui, L. H., Oh, T. R., Kim, J. H., Kim, T. W., & Kim, W. T. "Os BZR 1 turnover mediated by Os SK 22‐regulated U‐box E3 ligase Os PUB 24 in rice BR response" The Plant Journal 99(3):426-438 (2019).
  • You, D., Cho, Y. Y., Lee, H.-R., Lee, J.-H., Yu, S. J., Yoon, J.-H., … Lee, G. Y. "Hepatitis B virus X protein induces size-selective membrane permeabilization through interaction with cardiolipin" Biochimica et Biophysica Acta (BBA) - Biomembranes 1861(4):729–737 (2019). DOI: 10.1016/j.bbamem.2019.01.006.
This product has no review yet.
Browse Other Antibodies:
Select Rasied In:
Armenian hamster (0)
Bovine (0)
Chicken (0)
Donkey (0)
Goat (0)
Golden Syrian Hamster (0)
Guinea pig (0)
Hamster (0)
Horse (0)
Human (0)
Mouse (0)
Other (0)
Rabbit (0)
Rat (0)
Sheep (0)
Select Reactivity:
Avian (0)
Bombyx mori (0)
Bovine (0)
C. elegans (0)
Chicken (0)
CMV (0)
Dog (0)
Drosophila (0)
E. coli (0)
Fusarium sepsis (0)
Hamster (0)
HBV (0)
Horse (0)
Human (0)
Llama (0)
Locusta migratoria (0)
Manduca (0)
Mosquito (0)
Mouse (0)
Notapplicable (0)
Other (0)
Pig (0)
Planaria (0)
Porcine (0)
Quail (0)
Rat (0)
Rodent (0)
Sheep (0)
Species independent (0)
Spodoptera frugiperda (0)
Xenopus (0)
Zebrafish (0)