Anti-His Tag Antibody (Mouse Monoclonal)
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
|Unit quantity||100 μg|
|Specificity||His-tag fusion protein|
|Immunogen||6x His synthetic peptide.|
WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users.
|Recommended Dilutions||WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users.|
|Concentration||100 µg/100 µl|
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
|Storage Buffer||PBS, pH 7.4 with 0.05% sodium azide.|
|Cellular Localization||Depends upon the localization of the parent protein tagged with hexahistidine.|
abm guarantees that all our Anti-His Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.
|What is recommended antibody dilution as I will be conducting western blots shortly with this antibody (G020).|
The recommended starting dilution for this antibody is 1:1000. Of course, dilutions will be dependent on the sample and it should be optimized by the end user.
|Do you which species this antibody detects?|
This antibody will work on all antigens from different species provided that the antigen contains a His tag.
|What is the best dilution for detection of His using this ab?|
Optimal dilutions are to be determined by the end user. The recommended starting western blot dilution is 1:1000.
|What is the purification method for the tag antibodies?|
We use Protein A affinity beads for all tag antibody purification.
|What is the suggested starting dilution for western blots?|
We suggest a starting dilution of 1:1000.
|What is the expected size that will be obtained when SDS-PAGE is performed? i.e. What is the molecular weight of this antibody?|
For G020, you should be able to see two bands: one at 50kDa (which is the heavy chain), and one at the 25kDa (which is the light chain). The total molecular weight is 2x heavy chain + 2x light chain which is 150kDa in total.
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