Anti-His Tag Antibody (Mouse Monoclonal)

G020100 μg


DescriptionThe H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Unit quantity100 μg
Raised inMouse
Species ReactivityOther
SpecificityHis-tag fusion protein
Immunogen6x His synthetic peptide.

WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users.

Recommended DilutionsWB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users.
Storage Condition

This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Storage BufferPBS, pH 7.4 with 0.05% sodium azide.
Cellular LocalizationDepends upon the localization of the parent protein tagged with hexahistidine.

abm guarantees that all our Anti-His Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement.

PurityIgG fraction

Supporting Protocol




        What is recommended antibody dilution as I will be conducting western blots shortly with this antibody (G020).
        The recommended starting dilution for this antibody is 1:1000. Of course, dilutions will be dependent on the sample and it should be optimized by the end user.
        Do you which species this antibody detects?
        This antibody will work on all antigens from different species provided that the antigen contains a His tag.
        What is the best dilution for detection of His using this ab?
        Optimal dilutions are to be determined by the end user. The recommended starting western blot dilution is 1:1000.
        What is the purification method for the tag antibodies?
        We use Protein A affinity beads for all tag antibody purification.
        What is the suggested starting dilution for western blots?
        We suggest a starting dilution of 1:1000.
        What is the expected size that will be obtained when SDS-PAGE is performed? i.e. What is the molecular weight of this antibody?
        For G020, you should be able to see two bands: one at 50kDa (which is the heavy chain), and one at the 25kDa (which is the light chain). The total molecular weight is 2x heavy chain + 2x light chain which is 150kDa in total.

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