Anti-His Tag Antibody (Mouse Monoclonal)
Cat. No.
G020
Unit
100 μg
Price
$165.00
Specifications
| Cat. No. | G020 |
| Name | Anti-His Tag Antibody (Mouse Monoclonal) |
| Clone | HIS.H8 |
| Category | Tag Antibodies |
| Application | WB,IHC,IP,ICC/IF |
| Raised In | Mouse |
| Clonality | Tag |
| Reactivity | Other |
| Description |
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
| Immunogen | 6x His synthetic peptide. |
| Isotype | IgG2b |
| Concentration | 100 µg/100 µl |
| Recommended Dilutions | WB (with ECL): 1:1,000-3,000 dilution (incubate for one hour at room temperature). IS: 1:500-2000 dilution. Other applications are optimized by end users. |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Storage Condition |
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Specificity | His-tag fusion protein |
| Purity | IgG fraction |
| Guarantee |
abm guarantees that all our Anti-His Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
Protocols
There are no Protocols for this product yet!
Documents
FAQs
| What is recommended antibody dilution as I will be conducting western blots shortly with this antibody (G020). | |
|
The recommended starting dilution for this antibody is 1:1000. Of course, dilutions will be dependent on the sample and it should be optimized by the end user.
|
| Do you which species this antibody detects? | |
|
This antibody will work on all antigens from different species provided that the antigen contains a His tag.
|
| What is the best dilution for detection of His using this ab? | |
|
Optimal dilutions are to be determined by the end user. The recommended starting western blot dilution is 1:1000.
|
| What is the suggested starting dilution for western blots? | |
|
We suggest a starting dilution of 1:1000.
|
| What is the expected size that will be obtained when SDS-PAGE is performed? i.e. What is the molecular weight of this antibody? | |
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For G020, you should be able to see two bands: one at 50kDa (which is the heavy chain), and one at the 25kDa (which is the light chain). The total molecular weight is 2x heavy chain + 2x light chain which is 150kDa in total.
|
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