Idiopathic Autism Spectrum Disorder Fibroblastic Cell Line (sc204)

CAT.NOUNITPRICE
T63615x105 cells / 1.0 ml
$0.00

Specifications


Description

Idiopathic Autism Spectrum Disorder Fibroblastic Cell Line (sc204) was isolated from a patient who is negative for the FMR1 mutation or any other identifiable causes of Austism. Autism spectrum disorders (ASD) are poorly understood. A new panel of Idiopathic Autism Spectrum Disorder Fibroblastic Cell Lines are now available at abm for research needs. These will provide the ability to evaluate and compare data from a number of different cell lines which will facilitate greater insight into the cause of the ASDs and will be extremely useful for uncovering new therapeutic and diagnostic targets.

SKUT6361
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemSkin
Donor GenderMale
Donor Age8 years old
Donor DiseaseIdiopathic Autism Spectrum Disorder
Donor EthnicityCaucasian
Growth PropertiesAdherent
Cell MorphologyFibroblast
Applications

For Research Use Only

Unit quantity5x105 cells / 1.0 ml
Cell TypeDrug Discovery Cell Lines
Propagation Requirements

Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add fetal bovine serum (TM999) to a final concentration of 10%, MEM non-essential amino acids solution (Gibco) to a final concentration of 1%, GlutaMAX (Gibco) to a final concentration of 1% and penicillin-streptomycin solution (G255) to a final concentration of 1%,
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Subculture Protocol

1. Pre-warm the complete growth media specified by the cell line datasheet in a 37°C water bath. Pre-warm the Trypsin-EDTA (TM050) to room temperature.
2. Carefully aspirate the culture media from the culture vessel without disturbing the cell monolayer.
3. Add pre-warmed Trypsin-EDTA to the culture vessel. Gently rock the culture vessel to ensure complete coverage of the Trypsin-EDTA over the cells.
4. Observe the cells under a microscope to confirm they are dissociating from each other and are rounding up. Gently tap the culture vessel from several sides to promote cell detachment. Cells that are difficult to detach can be put in 37°C for
several minutes to facilitate dispersal.
5. When majority of the cells have detached, add an equal volume of the complete media into the culture vessel to neutralize the trypsin-EDTA. Gently swirl or pipette the culture suspension to ensure the neutralization is complete.
6. Transfer the culture suspension to a sterile centrifuge tube.
7. Centrifuge the cell suspension at 1500 rpm for 3 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
8. Aspirate the supernatant after checking all cells are pulled down into the pellet. Re-suspend the cell pellet in pre-warmed fresh complete media.
9. Pre-warm new culture vessels to 37°C. Seed cells at the recommended seeding density.
10. Place the newly seeded culture vessel in a 37°C, 5% CO2 incubator. Incubate for at least 24 – 48 hours before processing the cells for downstream experiments.
11. Renew the culture media every 2-3 days if the cells have not reached 80% confluency.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
DepositorInnovation Lab, LLC
Note

Brick, D. J., Nethercott, H. E., Montesano, S., Banuelos, M. G., Stover, A. E., Schutte, S. S., … Schwartz, P. H. (2014). The Autism Spectrum Disorders Stem Cell Resource at Children’s Hospital of Orange County: Implications for Disease Modeling and Drug Discovery. STEM CELLS Translational Medicine, 3(11), 1275–1286. doi: 10.5966/sctm.2014-0073.

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