Idiopathic Autism Spectrum Disorder Fibroblastic Cell Line (sc229)

CAT.NOUNITPRICE
T63635x105 cells / 1.0 ml
$0.00

Specifications


Description

Idiopathic Autism Spectrum Disorder Fibroblastic Cell Line (sc229) was isolated from a patient who is negative for the FMR1 mutation or any other identifiable causes of Austism. Autism spectrum disorders (ASD) are poorly understood. A new panel of Idiopathic Autism Spectrum Disorder Fibroblastic Cell Lines are now available at abm for research needs. These will provide the ability to evaluate and compare data from a number of different cell lines which will facilitate greater insight into the cause of the ASDs and will be extremely useful for uncovering new therapeutic and diagnostic targets.

SKUT6363
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemSkin
Donor GenderMale
Donor Age8 years old
Donor DiseaseIdiopathic Autism Spectrum Disorder
Donor EthnicityCaucasian
Growth PropertiesAdherent
Cell MorphologyFibroblast
Seeding Density10,000 - 20,000 cells/cm2
Population Doubling Time66 - 76 hours
Applications

For Research Use Only

Unit quantity5x105 cells / 1.0 ml
Cell TypeDrug Discovery Cell Lines
Propagation Requirements

Use of PriCoatTM T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add fetal bovine serum (TM999) to a final concentration of 10%, MEM non-essential amino acids solution (Gibco) to a final concentration of 1%, GlutaMAX (Gibco) to a final concentration of 1% and penicillin-streptomycin solution (G255) to a final concentration of 1%,
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

Subculture Protocol

1. Pre-warm the complete growth media specified by the cell line datasheet in a 37°C water bath. Pre-warm the Trypsin-EDTA (TM050) to room temperature.
2. Carefully aspirate the culture media from the culture vessel without disturbing the cell monolayer.
3. Add pre-warmed Trypsin-EDTA to the culture vessel. Gently rock the culture vessel to ensure complete coverage of the Trypsin-EDTA over the cells.
4. Observe the cells under a microscope to confirm they are dissociating from each other and are rounding up. Gently tap the culture vessel from several sides to promote cell detachment. Cells that are difficult to detach can be put in 37°C for several minutes to facilitate dispersal.
5. When majority of the cells have detached, add an equal volume of the complete media into the culture vessel to neutralize the trypsin-EDTA. Gently swirl or pipette the culture suspension to ensure the neutralization is complete.
6. Transfer the culture suspension to a sterile centrifuge tube.
7. Centrifuge the cell suspension at 1500 rpm for 3 minutes. The actual centrifuge duration and speed may vary depending on the cell type.
8. Aspirate the supernatant after checking all cells are pulled down into the pellet. Re-suspend the cell pellet in pre-warmed fresh complete media.
9. Pre-warm new culture vessels to 37°C. Seed cells at the recommended seeding density.
10. Place the newly seeded culture vessel in a 37°C, 5% CO2 incubator. Incubate for at least 24 – 48 hours before processing the cells for downstream experiments.
11. Renew the culture media every 2-3 days if the cells have not reached 80% confluency.

Preservation Protocol1. Freeze Medium: Complete growth medium with 20% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.

2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

4. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

5. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

6. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

7. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."

DepositorInnovation Lab, LLC
Note

Brick, D. J., Nethercott, H. E., Montesano, S., Banuelos, M. G., Stover, A. E., Schutte, S. S., … Schwartz, P. H. (2014). The Autism Spectrum Disorders Stem Cell Resource at Children’s Hospital of Orange County: Implications for Disease Modeling and Drug Discovery. STEM CELLS Translational Medicine, 3(11), 1275–1286. doi: 10.5966/sctm.2014-0073.

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