Immortalized Human Pulmonary Microvascular Endothelial Cells
Cat. No.
T0491
Unit
1x106 cells / 1.0 ml
Price
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Specifications
Description

The pulmonary microvascular endothelial cells (PMEC) are isolated from human pulmonary arteries. The PMECs form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. HPAEC have been used for the study of vascular permeability and inflammatory responses. HPAEC in co-culture with HPASMC have been used as a model for pulmonary angiopathy.

Cat. No. T0491
Name Immortalized Human Pulmonary Microvascular Endothelial Cells
Unit 1x106 cells / 1.0 ml
Category Immortalized Cell Lines
Cell Type Immortalized Cells
Organism Human (H. sapiens)
Tissue Blood Vessel
Cell Morphology Polygonal
Growth Properties Adherent
Seeding Density 50,000 cells/cm2
Population Doubling Time 25-35 hours
Immortalization Method Serial passaging and transduction with recombinant lentiviruses carrying SV40 Large T antigen
Propagation Method
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.

The base medium for this cell line is Prigrow I medium available at abm, Cat. No. TM001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999)* to a final concentration of 10% and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Change media every 2-3 days.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
* Do not use heat-inactivated FBS for cell culture unless specified otherwise.

QC

Real Time PCR was used to quantify transgene expression.

Application

For Research Use Only

BioSafety II
Disclaimer
1. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected].

2. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final.

3. We recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping).

4. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information.

5. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate.

6. abm warrants that cell lines shall be viable upon initiation of culture for a period of thirty (30) days after shipment and that they shall meet the specifications on the applicable abm Material Product Information sheet, certificate of analysis, and/or catalog description. Such thirty (30) day period is referred to herein as the "Warranty Period."
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FAQs
References
  • Huo, Z., Kong, Y., Meng, M., Cao, Z., Zhou, Q. "Atorvastatin enhances endothelial adherens junctions through promoting VE_PTP gene transcription and reducing VE-cadherin-Y731 phosphorylation" Vascular Pharmacology 117:7-14 (2019). DOI: 10.1016/j.vph.2018.06.003.
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