Luc-Akt-PH Stable MCF7 Cell Line

CAT.NOUNITPRICE
T31601x106 cells / 1.0 ml
$0.00

Specifications


DescriptionLuc-Akt-PH Stable MCF7 Cell Line expressed the pleckstrin homology (PH) domain of Akt fused to Renilla luciferase. The cells are suitable for the screening and study of molecules on the PI3K/Akt pathway for diabetes and cancer-related research. This cell line is the can be used with the Luc-Akt-PH/YFP Stable MCF-7/B2 Stable Cell Line (available at abm, Cat. T3161) for experimental studies.
SKUT3160
SpeciesHuman (H. sapiens)
Species descriptionHomo sapiens
Tissue/Organ/Organ SystemBreast
Donor GenderFemale
Donor Age69-year Old
Donor EthnicityCaucasian
Growth PropertiesAdherent
Cell MorphologyPolygonal
Seeding DensityThaw entire contents into an appropriate T25 flask as specified in the Propagation instructions.
ApplicationsFor Research Use Only
Unit quantity1x106 cells / 1.0 ml
Pharmaceutical TargetEnzymes
CautionFor Research Use Only
Cell TypeDrug Discovery Cells
Propagation Requirements
Use of PriCoatTMT25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells inECM-coated culture vessels unless otherwise specified in the Propagation Requirements below.
The base medium for this cell line is Prigrow III medium available at abm, Cat. No. TM003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (TM999) to final concentration of 10%, 1 mg/ml geneticin (G271), and Penicillin/Streptomycin Solution (G255) to a final concentration of 1%.
Carbon dioxide (CO2): 5%, Temperature: 37.0°C.
QC1) Selection preformed with 1 mg/ml geneticin; 2) BRET assay utilized
Disclaimer

1. For for-profit organizations and corporations, please contact [email protected] for pricing of this item.
2. Sale of this item is subjected to the completion of a Material Transfer Agreement (MTA) by the purchasing individual/institution for each order. If you have any questions regarding this, please contact us at [email protected]
3. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5

DepositorIDF Innov
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References


1
  • Kuo, M et al. "Development of a Human Breast-Cancer Derived Cell Line Stably Expressing a Bioluminescence Resonance Energy Transfer (BRET)-Based Phosphatidyl Inositol-3 Phosphate (PIP3) Biosensor" PLoS One 9(3):e92737 (2014). DOI: 10.1371/journal.pone.0092737.