MAPRE1/MAPRE3 Doxycycline Inducible Double Knockout HeLa Cell Line
|T6174||1x106 cells / 1.0 ml|
|Description||This human cell line stably expresses single guide RNA (sgRNA) targeting an early exon of MAPRE1 (microtubule-associated protein RP/EB family member 1) and of MAPRE3 (microtubule-associated protein RP/EB family member 3). Cas9 expression is induced by the presence of doxycycline enabling inducible knockout of the genes of interest. A PiggyBac transposon system was used to introduce Cas9 while guide RNAs were introduced using lentiviral infection.|
|Species||Human (H. sapiens)|
|Applications||For Research Use Only|
|Seeding Density||40,000 - 50,000 cells/cm2|
|Population Doubling Time||24 - 34 hours|
|Cell Type||Drug Discovery Cell Lines|
|Expression Profile||EB1/MAPRE1 and EB3/MAPRE3; doxycycline-inducible Cas9 nuclease|
|Preservation Protocol||1. Freeze Medium: Complete growth medium with 50% FBS and 10% DMSO.|
2. Storage Temperature: Liquid nitrogen vapour phase.
|Unit quantity||1x106 cells / 1.0 ml|
|QC||1) Western blot; 2) Immunofluorescence|
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