MAPRE1/MAPRE3 Doxycycline Inducible Double Knockout HeLa Cell Line

CAT.NOUNITPRICE
T61741x106 cells / 1.0 ml
$0.00

Specifications


DescriptionThis human cell line stably expresses single guide RNA (sgRNA) targeting an early exon of MAPRE1 (microtubule-associated protein RP/EB family member 1) and of MAPRE3 (microtubule-associated protein RP/EB family member 3). Cas9 expression is induced by the presence of doxycycline enabling inducible knockout of the genes of interest. A PiggyBac transposon system was used to introduce Cas9 while guide RNAs were introduced using lentiviral infection.
SKUT6174
SpeciesHuman (H. sapiens)
Tissue/Organ/Organ SystemCervix
Cell MorphologyEpithelial-like
ApplicationsFor Research Use Only
Seeding Density40,000 - 50,000 cells/cm2
Population Doubling Time24 - 34 hours
Cell TypeDrug Discovery Cell Lines
Growth PropertiesAdherent
Expression ProfileEB1/MAPRE1 and EB3/MAPRE3; doxycycline-inducible Cas9 nuclease
Preservation Protocol1. Freeze Medium: Complete growth medium with 50% FBS and 10% DMSO.
2. Storage Temperature: Liquid nitrogen vapour phase.
Unit quantity1x106 cells / 1.0 ml
QC1) Western blot; 2) Immunofluorescence
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