Recombinant Human AREG (E. coli)

Cat. No.

Z101875

Unit

Price

$54.00

Cat. No.	Z101875
Name	Recombinant Human AREG (E. coli)
Description	Amphiregulin is a trans-membrane protein whose protease mediated digestion of the extracellular domain enables release of mature AR. Amphiregulin works to stimulate the proliferation of some animal keratinocyles, epithelial cells and fibroblasts. It has been shown that the mRNA expression of amphiregulin can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues, including colon, stomach, breast, ovary and kidney.
Organism	Human (H. sapiens)
Unit quantity	15 µg
Family	EGF Family
Tag	Tag Free
Aliases	AR, AREGB, CRDGF, SDGF
Gene Symbol	AREG
Gene ID	374
Accession Number	P15514
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	11.5 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein
Function	Measured in a cell proliferation assay using mouse embryonic fibroblast 3T3 cells. The ED(50) for this effect is <0.5 ng/mL.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101875

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.