Recombinant Human Artemin (ARTN) (E. coli)

Cat. No.
Z102355
Unit
Price
$205.00
Cat. No. Z102355
Name Recombinant Human Artemin (ARTN) (E. coli)
Description Artemin is a disulfide-linked member of the glial cell line-derived homodimeric neurotrophic factor (GDNF) family which all signal via a multicomponent receptor system composing of a high-affinity binding component (GFRα1-4) and a common signalling receptor tyrosine kinase (RET). Artemin prefers the receptor GFRα3-RET, but will use the others as an alternative. Artemin promotes the survival and growth of peripheral and central neurons, including dopaminergic neurons in the mid-brain; and plays an important role in the development of sensory and sympathetic neurons.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family TGF-beta Super-family
Aliases Enovin, Neublastin
Gene Symbol ARTN
Gene ID 9048
Accession Number Q5T4W7
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 12.2 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered 10mM sodium citrate pH-4.5 and 25mM sodium chloride solution
Function The ED50 as determined by its binding ability in a functional ELISA (using SH-SY5Y human neuroblastoma cells), is 4-16 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102355
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