Recombinant Human CCL2 (E. coli)

Cat. No.
Cat. No. Z100625
Name Recombinant Human CCL2 (E. coli)
Description MCP-1 belongs to the family of chemotactic cytokines. MCP-1 is expressed by monocytes, vascular endothelial cells, smooth muscle cells, glomerular mesangial cell, and osteoblastic cells. MCP-1 has been shown to exhibit biological activities other than chemotaxis. It can induce the proliferation and activation of killer cells known as CHAK. Elevated levels of MCP-1 are observed in atherosclerotic plaques that are rich in macrophages. The factor activates the tumoricidal activity of monocytes and macrophages in vivo. It regulates the expression of cell surface antigens (CD11c, CD11b) and the expression of cytokines (IL-1, IL-6). MCP-1 is a potent activator of human basophils, inducing degranulation and the release of histamines. In basophils activated by IL-3, IL-5 or GM-CSF MCP-1 enhances the synthesis of leukotriene C4.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family Chemokine-beta Family (CC)
Aliases Monocyte Chemotactic Protein-1, MCP-1, CCL2, MCAF, C-C motif chemokine 2, HC11, Monocyte chemotactic and activating factor, Monocyte secretory protein JE, Small-inducible cytokine A2, MCP1, SCYA2
Recombinant Human Monocyte Chemoattractant Protein-1 (CCL2)
Gene Symbol CCL2
Gene ID 6347
Accession Number P13500
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 9 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation MCP-1 was lyophilized from PBS pH 7.4.
Function The ED(50) was determined by the dose-dependent proliferation of PC3 cells and was found to be <0.2ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage This cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100625
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg