Recombinant Human CTGF (E. coli)

Cat. No.
Cat. No. Z102025
Name Recombinant Human CTGF (E. coli)
Description Secreted by vascular endothelial cells, CTGF is a regulatory protein that has been shown to stimulate the growth of chondrocytes, promote angiogenesis and mediate cell adhesion of fibroblasts and endothelial cells. The binding of CTGF to heparin induces cell migration and adhesion. Mature Human CTGF is a 38.0 kDa secreted protein that is comprised of four distinct structural domains (a) IGFBP N-terminal domain (b) VWFC domain (c) TSP type-I domain and (d) C-terminal CTCK domain. Recombinant Human CTGF is an 11.3 kDa protein monomeric protein that maps to the CTCK domain.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family CNTF/CCN Families
Aliases CTGF, CCN family member 2, Hypertrophic chondrocyte-specific protein 24, HCS24, Insulin-like growth factor-binding protein 8, IBP-8, IGF-binding protein 8, IGFBP-8
Recombinant Human Connective Tissue Growth Factor (CTGF)
Gene Symbol CTGF
Gene ID 1490
Accession Number P29279
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 11.3 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2µm filtered solution containing 0.1% TFA
Function Determined by the dose-dependent stimulation of the proliferation of HUVEC cells. The expected ED50 for this effect is 1.0-2.0 µg/ml.
Reconstitution A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102025
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg