Recombinant Human CXCL4 (PF4)

Cat. No.
Z101005
Unit
Price
$246.00
Cat. No. Z101005
Name Recombinant Human CXCL4 (PF4)
Description Platelet factor 4 (PF4/CXCL4) is an ELR− tetrameric, cationic chemokine that constitutes 25% of the protein in platelet alpha-granules. It is also found bound to the luminal vascular endothelial surface. Although platelets represent the primary source of PF4, it is also expressed at lower levels in other cells of the immune system including cultured T cells, monocytes, and endothelial and smooth muscle cells.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family Chemokine-alpha Family (CXC)
Aliases C-X-C motif chemokine 4, Iroplact, Oncostatin-A, CXCL4, SCYB4
Recombinant Human Platelet Factor 4 (PF4)
Gene Symbol PF4
Gene ID 5196
Accession Number P02776
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 8 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered solution containing 20 mM PBS,1.5 M NaCl pH 7.4
Function Determined by its ability to inhibit human FGF-basic-dependent proliferation of NR6R-3T3 mouse fibroblasts. The ED50 for this effect is typically 5-15 μg/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101005
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