Recombinant Human EGFL7

Cat. No.

Z101535

Unit

25 µg

Price

$960.00

Cat. No.	Z101535
Name	Recombinant Human EGFL7
Description	EGFL7 is an 31 kDa secreted protein that contains an Emilin-like (EMI) domain, and two epidermal growth factor (EGF) domains - one of which binds calcium. Based on these domains, it has been hypothesized that EGFL7 may self-assemble, similar to extracellular matrix (ECM) proteins and, thus, incorporate into ECM. EGFL7 has been reported to stimulate cell adhesion as well as motility in a manner similar to ECM proteins. EGFL7 has been shown to be primarily expressed by developing ECs but also by primordial germ cells and some central nervous system neurons. Interestingly, EGFL7 expression markedly decreases in ECs in postnatal life, but can be strongly up-regulated after various tissue injuries that lead to increased angiogenic responses.
Organism	Human (H. sapiens)
Unit quantity	25 µg
Family	EGF Family
Tag	Tag Free
Aliases	NEU1, VE-STATIN, ZNEU1
Gene Symbol	EGFL7
Gene ID	51162
Accession Number	Q9UHF1
Source	Insect cells
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	31 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant Human EGFL7 is lyophilized from a 0.2 μm filtered solution (pH 7.0) in 100 mM glycine, 10 mM NaCl, 10% glycerol and 5% trehalose.
Function	The activity was determined by the ability to bind human NOTCH1 in functional ELISA.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101535

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.