Recombinant Human ENA-78 (CXCL5)

Cat. No.
Cat. No. Z100165
Name Recombinant Human ENA-78 (CXCL5)

Epithelial neutrophil- activating protein 78 (ENA-78) is a member of the CXC chemokines and acts as a potent chemoattractant and activator of neutrophil function. On stimulation in vitro, ENA-78 is highly expressed in many cell types. ENA-78 protein levels are strongly elevated in synovial fluid and blood of patients with rheumatoid arthritis. By in situ hybridization and immunofluorescence staining, ENA-78 has been recognized as a major CXC chemokine expressed in epithelial cells of the intestinal mucosa of patients with Crohn's disease, ulcerative colitis, and acute appendicitis.

Organism Human (H. sapiens)
Unit quantity 20 µg
Family Chemokine-alpha Family (CXC)
Aliases ENA78(1-78), Epithelial-derived neutrophil-activating protein 78, Neutrophil-activating peptide ENA-78, Small-inducible cytokine B5, ENA78, SCYB5, CXCL5
Gene Symbol CXCL5
Accession Number P42830
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 8 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.

>95% as determined by SDS-PAGE

Formulation Lyophilized from a 0.2 µm filtered solution in 20 mM PB, 100 mM NaCl solution pH 7.5.
Function Determined by its ability to chemoattract human neutrophils using a concentration range of 2.0 - 40.0 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100165
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