Recombinant Human FGF21 (E. coli)

Cat. No.
Z101105
Unit
Price
$89.00
Cat. No. Z101105
Name Recombinant Human FGF21 (E. coli)
Description FGF21 is a secreted growth factor of the FGF family, classified as an FGF19 subfamily member. Proteins of the FGF family are known to play a central role with respect to both prenatal and postnatal growth and development, and regeneration of a variety of tissues by promoting cellular proliferation and differentiation. In the presence of beta Klotho (protein cofactor), FGF21 signals via the FGFR receptors to stimulate insulin independent glucose uptake by adipocytes.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family FGF Family
Aliases FGF-21
Recombinant Human Fibroblast Growth Factor 21 (FGF21)
Gene Symbol FGF21
Gene ID 26291
Accession Number Q9NSA1
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 19.7 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from 0.2 μm filtered solution in Tris and NaCl (pH 8.0)
Function The bioactivity was determined in a NIH/3T3 cell proliferation assay with an ED50 <1ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101105
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