Recombinant Human FGF3 (E. coli)

Cat. No.
Z102285
Unit
20 µg
Price
$152.00
Cat. No. Z102285
Name Recombinant Human FGF3 (E. coli)
Description FGF3 belongs to the FGF family, members of which act on cells of mesodermal and neuroectodermal origin. Originally designated int2, the FGF3 gene was initially identified as a protooncogene in mice. FGF3 plays an important role in the regulation of embryonic development, cell proliferation, and cell differentiation and is required for normal ear development. Heparan sulfate glycosaminoglycans function as co-receptors to increase the affinity between FGF3 and its receptors; FGFR1 and FGFR2. Human and mouse FGF3 are known to share 88% amino acid sequence identity. Recombinant Human FGF3 is a single polypeptide of 21 kDa.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family FGF Family
Aliases HBGF-3, FGF3, Proto-oncogene Int-2, Heparin-binding growth factor 3
Gene Symbol FGF3
Gene ID 2248
Accession Number P11487
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 21.2 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in PBS, DTT and EDTA
Function The ED50 as determined by the dose-dependent stimulation of mouse fibroblasts was <5ng/ml
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102285
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