Recombinant Human FGF8 (E. coli)

Cat. No.
Z101705
Unit
Price
$89.00
Cat. No. Z101705
Name Recombinant Human FGF8 (E. coli)
Description FGF-8 is a heparin binding protein of the FGF family which works to control cell division, differentiation, and function. These proteins are especially prominent during prenatal development and postnatal growth. FGF-8 can be spliced in four different ways. The products of the FGF-8 splicing events are as follows: FGF8A, 8B, 8E and 8F. This recombinant product represents isoform 8B.
Organism Human (H. sapiens)
Unit quantity 50 µg
Family FGF Family
Aliases FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8
Recombinant Human Fibroblast Growth Factor 8 (FGF8)
Gene Symbol FGF8
Gene ID 2253
Accession Number P55075
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 22.5 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in 20mM phosphate buffer, 0.2 mM EDTA and 1M NaCl (pH 8.0)
Function The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101705
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