Recombinant Human G-CSF (CSF3) (E. coli)

Cat. No.
Cat. No. Z100245
Name Recombinant Human G-CSF (CSF3) (E. coli)
Description G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is also produced by stromal cells, fibroblasts, endothelial cells, epithelial carcinomas, acute myeloid leukemia cells, and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM-CSF. Comparison of the primary sequence of G-CSF with those of GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF share a sequence homology (protein) of approximately 70% and exhibit cross reactivity. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family IL-6 Family
Aliases GCSF, CSF-3, MGI-1G, Pluripoietin, Human Granulocyte Colony Stimulating Factor (CSF3)
Gene Symbol CSF3
Gene ID 1440
Accession Number P09919
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 19 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl (pH 7.5)
Function The ED50 as determined by the dose-dependent proliferation of neuroblastoma cell line was found to be ≤5ng/ml
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100245
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg