Recombinant Human GDF3 (E. coli)

Cat. No.
Z101735
Unit
Price
$205.00
Cat. No. Z101735
Name Recombinant Human GDF3 (E. coli)
Description GDF-3 is a growth and differentiation factor which belongs to the TGF-beta superfamily. GDF-3 and GDF-9 are the only two members of the family which do not form disulfide-linked dimers. GDF-3 has been found in adult bone marrow, spleen, thymus, and adipose tissue. Although signaling in all other members of the family involve the BMP receptors ALK1,2,3, and 6, GDF-3 signals through BMP receptors ALK4 and 7.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family TGF-beta Super-family
Aliases GDF-3
Recombinant Human Growth Differentiation Factor 3 (GDF3)
Gene Symbol GDF3
Gene ID 9573
Accession Number Q9NR23
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 13 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from solution in 30mM Acetate buffer, pH 4
Function Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.
Reconstitution A quick spin of the vial followed by reconstitution in sterile 100mM Acetate buffer, pH 4 at a concentration of 0.5 mg/mL. Please note that in higher concentrations the solubility of GDF3 is limited. The protein is not lypholized in a filtered solution. Please sterile filter before using it in the cell culture.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101735
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