Recombinant Human GH1 (E. coli)
| Cat. No. | Z100265 |
| Name | Recombinant Human GH1 (E. coli) |
| Description |
The physiological activity for which growth hormone is best known is the promotion of growth of bone, cartilage, and soft tissues. Detectable levels of growth hormone are found throughout the remainder of adulthood, suggesting other functions in addition to promotion of growth. Growth hormone may be important for the maintenance of lean body mass; most growth-promoting effects of growth hormone are mediated by IGF-1 the synthesis of which is regulated by growth hormone. The biological activities of growth hormone are mediated by receptors belonging to one of the cytokine receptor families. Growth hormone has been shown to be produced by T cells, B cells, and macrophages. In human lymphocytes growth hormone appears to up-regulate its own expression. Growth hormone appears to act as an enhancer of immune responses and is produced in considerable amounts by T helper cells. |
| Organism | Human (H. sapiens) |
| Unit quantity | 500 µg |
| Family | Somatotropin/Sclerostin/Serpin Families |
| Tag | Tag Free |
| Aliases |
GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N |
| Gene Symbol | GH1 |
| Gene ID | 2688 |
| Accession Number | P01241 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 22 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
| Purity |
>95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered in Tris and NaCl (pH 7.5). |
| Function | The ED(50) was determined by the dose-dependent cell proliferation assay using the rat lymphoma, Nb2-11, and was found to be in the range of 0.1 ng/mL. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Shipping Conditions | This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions. |
| Storage | Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100265 |
| How do I choose the right growth factor for my experiment? | |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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