Recombinant Human GM-CSF (CSF2) (E. coli)

Cat. No.
Z100275
Unit
Price
$89.00
Cat. No. Z100275
Name Recombinant Human GM-CSF (CSF2) (E. coli)
Description GM-CSF is a hematopoietic growth factor that is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes following their activation by antigens or mitogens. GM-CSF stimulates the development of macrophages and neutrophils and plays a role in the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. Approximately 90% of the secreted colony stimulating activities are due to GM-CSF. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo compared to its non-glycosylated counterpart. Human and murine GM-CSF are species-specific and therefore, exhibit no cross-reactivity. Recombinant Human GM-CSF is a non-glycosylated, monomeric protein that contains intra-chain disulfide bonds.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family Other Growth Factors/Cytokines
Aliases GM-CSF, Granulocyte-macrophage colony-stimulating factor, CSF-2, Pluripoietin-alpha, Recombinant Human Granulocyte Macrophage Colony Stimulating Factor (CSF2)
Gene Symbol CSF2
Gene ID 1437
Accession Number P04141
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 15 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl
Function The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells was found to be ≤ 0.5 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100275
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