Recombinant Human GMFB (E. coli)
Cat. No. | Z101115 |
Name | Recombinant Human GMFB (E. coli) |
Description | GMF-beta is a 17-kDa brain-specific protein that was isolated from bovine brain homogenate as a substance inducing the maturation of normal neurons as well as glial cells, and at first, it was considered to be a neurotrophic factor. The amino acid sequence of GMFB is highly conserved among many species, suggesting that it plays basic roles across many species. The expression of GMF-b is largely limited to the brain, especially the glial cells and some neurons. Schwann cells of the distal segment of the transected nerve express GMF-b, and this induction of GMF-b coincides with the temporal expression of nerve growth factor receptors in the cell. |
Organism | Human (H. sapiens) |
Unit quantity | 10 µg |
Family | Other Growth Factors/Cytokines |
Aliases |
GMF-beta, GMFB Recombinant Human Glia Maturation Factor Beta (GMFB) |
Gene Symbol | GMFB |
Gene ID | 2764 |
Accession Number | P60983 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 15 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
Purity | >95% as determined by SDS-PAGE |
Formulation | Recombinant GMFB was lyophilized from a 0.2 μm filtered 20 mM PB,130 mM NaCl solution pH 7.5. |
Function | ED50 not determined. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101115 |
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