Recombinant Human IGF1 (E. coli)
Cat. No. | Z100385 |
Name | Recombinant Human IGF1 (E. coli) |
Description | The Insulin Growth Factors belong to the Insulin gene family, the prototypic members of which include insulin and relaxin. Also known as somatomedin C, IGF1 is known to stimulate the proliferation of a wide range of cells including muscle, bone, and those of the cartilage tissue. A dominant effector of the growth hormone, mature IGF1 is generated by proteolytic removal of the N- and C- terminal pro-peptide regions. Mature human IGF1 shares 94% and 96% amino acid sequence identity with mouse and rat IGF1, respectively, while exhibiting cross-species activity. Circulating IGF1 is produced by hepatocytes while local IGF1 is produced by various other tissues in which it exerts paracrine effects. Recombinant Human IGF1 is a globular, 7.7 kDa protein that contains 3 native intra-molecular disulfide bonds. |
Organism | Human (H. sapiens) |
Unit quantity | 100 µg |
Family | IGF/Insulin Family |
Aliases |
IGF-I, Mechano growth factor, MGF, Somatomedin-C, IBP1 Recombinant Human Insulin Like Growth Factor-I (IGF1) |
Gene Symbol | IGF1 |
Gene ID | 3479 |
Accession Number | P01343 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 7.7 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
Purity | >95% as determined by SDS-PAGE |
Formulation | Lyophilized from a 0.2 μm filtered solution in sodium phosphate (pH 6.5) and 150mM NaCl |
Function | The ED50 as determined by a cell proliferation assay using MCF-7 cells was found to be ≤20 ng/ml |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100385 |
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