Recombinant Human IL18 (E. coli)

Cat. No.

Z100475

Unit

Price

$92.00

Cat. No.	Z100475
Name	Recombinant Human IL18 (E. coli)
Description	Also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, IL18 is a cytokine that bears structural similarities with members of the IL-1 family of proteins. IL18 is natively synthesized as a proform that gets processed by caspase-1 to generate the bioactive form of IL18. IL18 is one of the pro-inflammatory cytokines that is expressed by a variety of immune and non-immune cells. The activities of IL18 appear to be species specific. An important function of IL18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses. IL18 functions as a growth and differentiation factor for Th1 cells and is known to up-regulate the FAS ligand mediated cytotoxic activity of murine natural killer cells. IL18 also partakes in a complex regulatory circuit involved in causing cell death by apoptosis.
Organism	Human (H. sapiens)
Unit quantity	25 µg
Family	IL-1 Family
Tag	Tag Free
Aliases	IGIF, IL-18, IL-1g, IL1F4
Gene Symbol	IL18
Gene ID	3606
Accession Number	Q14116
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	18.5 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from 0.2 μm filtered solution in Tris buffer with NaCl
Function	The ED(50) as determined by the dose-dependent proliferation of human PBMC co-stimulated with IL12 was found to be in the range of 5.0 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100475

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.