Recombinant Human IL18 (E. coli)

Cat. No.
Cat. No. Z100475
Name Recombinant Human IL18 (E. coli)
Description Also known as interferon-gamma-inducing factor (IGIF) and IL-1γ, IL18 is a cytokine that bears structural similarities with members of the IL-1 family of proteins. IL18 is natively synthesized as a proform that gets processed by caspase-1 to generate the bioactive form of IL18. IL18 is one of the pro-inflammatory cytokines that is expressed by a variety of immune and non-immune cells. The activities of IL18 appear to be species specific. An important function of IL18 is the regulation of functionally distinct subsets of T-helper cells required for cell mediated immune responses. IL18 functions as a growth and differentiation factor for Th1 cells and is known to up-regulate the FAS ligand mediated cytotoxic activity of murine natural killer cells. IL18 also partakes in a complex regulatory circuit involved in causing cell death by apoptosis.
Organism Human (H. sapiens)
Unit quantity 25 µg
Family IL-1 Family
Aliases IL-18, Interferon gamma-inducing factor, IFN-gamma-inducing factor, Interleukin-1 gamma, IL-1 gamma
Recombinant Human Interleukin-18 (IL18)
Gene Symbol IL18
Gene ID 3606
Accession Number Q14116
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 18.5 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from 0.2 μm filtered solution in Tris buffer with NaCl
Function The ED(50) as determined by the dose-dependent proliferation of human PBMC co-stimulated with IL12 was found to be in the range of 5.0 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100475
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg