Recombinant Human IL1A (E. coli)

Cat. No.
Cat. No. Z100405
Name Recombinant Human IL1A (E. coli)
Description There are two functionally comparable forms of IL1: IL1A and IL1B, that are encoded by two different genes. IL1B is the predominant form in humans while, it is IL1A in mice. Both forms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. Latter include but not limited to, stimulation of T-helper cells, promotion, proliferation and synthesis of immunoglobulins and the activation of fibroblasts and NK cells. Unlike IL1B, the IL1A precursor does not require processing before it can bind to the receptor. Monocytes are the main source of secreted IL1B while human keratinocytes are known to express large amounts of IL1A. Recombinant Human IL1A is a non-glycosylated polypeptide of 18 kDa.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family IL-1 Family
Aliases Hematopoietin-1, Lymphocyte-activating factor, LAF, Mononuclear Cell Factor, MCF, IL1F1
Recombinant Human Interleukin-1 Alpha (IL1A)
Gene Symbol IL1A
Gene ID 3552
Accession Number P01583
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 18 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl (pH 8.0)
Function The ED50 as determined by the dose-dependent stimulation of murine D10S cells was found to be ≤ 0.002 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100405
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg