Recombinant Human IL1B (E. coli)

Cat. No.
Z100395
Unit
Price
$246.00
Cat. No. Z100395
Name Recombinant Human IL1B (E. coli)
Description Monocytes are the main source of secreted IL1; expressing mostly IL1B, while human keratinocytes express large amounts of IL1A. Overall, IL1B is the predominant form in humans while, IL1A is the one in mice. Both isoforms of IL1 bind to the same receptor and therefore show similar, if not identical, biological activities. The IL1B precursor though must be processed before it can bind to the receptor. IL1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. A monomer, IL1B stimulates thymocyte proliferation, B-cell maturation and proliferation and fibroblast growth factor activity.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family IL-1 Family
Aliases Catabolin, Lymphocyte-activating factor, LAF, Endogenous Pyrogen, EP, IL1F2
Recombinant Human Interleukin-1 Beta (IL1B)
Gene Symbol IL1B
Gene ID 3553
Accession Number P01584
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 17.5 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, EDTA and DTT (pH 8.0)
Function The ED50 as determined by the dose-dependent inhibition of prostate carcinoma cell line is less than 1ng/ml
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100395
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