Recombinant Human IL1RN (E. coli)

Cat. No.
Z100415
Unit
Price
$89.00
Cat. No. Z100415
Name Recombinant Human IL1RN (E. coli)
Description IL1RN is found in the conditioned medium of a variety of cells and is produced by macrophages, monocytes, and neutrophils. The soluble form of IL1RN has been shown to be produced by hepatocytes and to be regulated by pro-inflammatory cytokines. It may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease.
Organism Human (H. sapiens)
Unit quantity 100 µg
Family IL-1 Family
Aliases IRAP, ICIL-1RA, IL1 inhibitor, IL1F3, IL-1ra, IL-1RN
Recombinant Human Interleukin-1 Receptor Antagonist (IL1RN)
Gene Symbol IL1RN
Gene ID 3557
Accession Number P18510
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 17 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered tris solution with sodium chloride (pH 7.5)
Function The ED50 was determined by the ability to inhibit the IL-1 alpha mediated cell proliferation in a murine helper T cell line, for this effect, in the presence of 50 pg/mL of IL-1 alpha, is typically 20-60 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100415
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