Recombinant Human IL3 (E. coli)
Cat. No. | Z100515 |
Name | Recombinant Human IL3 (E. coli) |
Description | IL3 is produced mainly by T cells following cell activation by antigens and mitogens, but also by keratinocytes, natural killer cells, mast cells, endothelial cells, and monocytes. The analysis of bacterial- derived recombinant IL3 shows that glycosylation is not required for activity. IL3 sequences are evolutionarily less well conserved with human and murine IL3 sharing approximately 29% homology (at the protein level) and murine and rat IL3 sharing approximately 54% homology. IL3 receptors are expressed on macrophages, mast cells, eosinophils, megakaryocytes, basophils, bone marrow progenitor cells and various myeloid leukemia cells. Binding of IL3 to its receptor causes specific phosphorylation of a 150 kDa membrane glycoprotein. Recombinant human IL3 is a non-glycosylated globular protein. |
Organism | Human (H. sapiens) |
Unit quantity | 10 µg |
Family | Other Interleukins |
Aliases |
IL-3, Mast cell growth factor, MCGF, Multi-CSF, Hematopoietic growth factor, HCGF, P-cell stimulation factor Recombinant Human Interleukin-3 (IL3) |
Gene Symbol | IL3 |
Gene ID | 3562 |
Accession Number | P08700 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 15 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
Purity | >95% as determined by SDS-PAGE |
Formulation | Lyophilized from a 0.2 μm filtered solution in Phosphate Buffer and NaCl (pH 6.3) |
Function | The ED50 as determined by the dose-dependent proliferation of Human TF-1 cells was found less than 0.5 ng/mL |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100515 |
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