Recombinant Human IL7 (E. coli)

Cat. No.
Z100975
Unit
Price
$89.00
Cat. No. Z100975
Name Recombinant Human IL7 (E. coli)
Description IL7 is a secreted cytokine of the hemopoietin family that primarily affects early B and T cells and plays an important role in stimulating the proliferation of lymphoid progenitors. IL7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. Human and murine IL7 share 60% sequence homology at the primary level and known to exhibit cross species activity. Designated CD127, the human IL7 receptor is a strongly glycosylated membrane protein of 76 kDa. Recombinant Human IL7 is a 17.7 kDa, non-glycosylated protein.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family Other Interleukins
Aliases IL-7
Recombinant Human Interleukin-7 (IL7)
Gene Symbol IL7
Gene ID 3574
Accession Number P13232
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 17.7 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in sodium phosphate and NaCl
Function The ED50 as determined by dose-dependent stimulation of mouse lymphoma was <0.5ng/ml
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100975
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