Recombinant Human Leptin (LEP) (E. coli)

Cat. No.
Z101855
Unit
Price
$84.00
Cat. No. Z101855
Name Recombinant Human Leptin (LEP) (E. coli)
Description Leptin is a cytokine found in the adipose tissue of animals (Leptin mRNA is restricted to the adipose tissue). Leptin has been shown to suppress appetite, increase thermogenesis and reduce body weight, food consumption and plasma glucose levels.
Organism Human (H. sapiens)
Unit quantity 200 µg
Family FIZZ(Resistin)/Leptin Families
Aliases OB, Obese protein, Obesity factor
Gene Symbol LEP
Gene ID 3952
Accession Number P41159
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 16.4 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Function The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.
Reconstitution Reconstitute at 1 mg/mL in sterile 20 mM Tris-­HCI (pH 8.0).
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101855
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