Recombinant Human Leptin (LEP) (E. coli)

Cat. No.

Z101855

Unit

Price

$87.00

Cat. No.	Z101855
Name	Recombinant Human Leptin (LEP) (E. coli)
Description	Leptin is a cytokine found in the adipose tissue of animals (Leptin mRNA is restricted to the adipose tissue). Leptin has been shown to suppress appetite, increase thermogenesis and reduce body weight, food consumption and plasma glucose levels.
Organism	Human (H. sapiens)
Unit quantity	200 µg
Family	FIZZ(Resistin)/Leptin Families
Tag	Tag Free
Aliases	LEPD, OB, OBS
Gene Symbol	LEP
Gene ID	3952
Accession Number	P41159
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	16.4 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Function	The ED(50) was determined by the dose-dependent proliferation of human umbilical vein endothelial cells and was found to be <0.5ng/mL.
Reconstitution	Reconstitute at 1 mg/mL in sterile 20 mM Tris-HCI (pH 8.0).
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101855

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.