Recombinant Human LIF (E. coli)

Cat. No.
Z100605
Unit
Price
$89.00
Cat. No. Z100605
Name Recombinant Human LIF (E. coli)
Description LIF is a multifunctional secreted glycoprotein that exists in both soluble and matrix bound forms. It displays biologic activities ranging from the differentiation of myeloid leukemic cells into macrophage lineage to effects on bone metabolism, inflammation, neural development, embryogenesis, and the maintenance of implantation. It is now clear that LIF is related in both structure and mechanism of action to the interleukin IL-6 family of cytokines, which also includes IL-11, ciliary neurotrophic factor, oncostatin M, and cardiotrophin 1. The actions of these cytokines are mediated through specific cell-surface receptors that consist of a unique chain and the shared signal transducing subunit gp130.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family MIA(OTOR)/MIF/OSM(LIF) Families
Aliases LIF, Differentiation-stimulating factor, D factor, Melanoma-derived LPL inhibitor (MLPLI)
Recombinant Human Leukemia Inhibitory Factor (LIF)
Gene Symbol LIF
Gene ID 3976
Accession Number P15018
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 20 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered PBS pH 7.0
Function The ED50 was in found to be <0.05 ng as determined by the dose-dependent proliferation of human erythroleukemic cell line.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100605
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