Recombinant Human M-CSF (CSF1) (E. coli)

Cat. No.
Cat. No. Z100655
Name Recombinant Human M-CSF (CSF1) (E. coli)
Description M-CSF is produced by monocytes, granulocytes, endothelial cells, and fibroblasts. After cell activation, B cells and T cells and also a number of tumor cell lines are capable also of synthesizing this factor. M-CSF has been found to be synthesized by uterine epithelial cells in vivo. The M-CSF receptor is identical with the proto-oncogene fms. It has been renamed CD115. The receptor is a transmembrane protein with an extracellular ligand-binding domain of 512 amino acids, an intramembrane domain of 25 amino acids, and a cytoplasmic domain of 435 amino acids encoding a tyrosine kinase. Human M-CSF is active in mouse and rat cells. The murine factor is active in rat cells but inactive in human cells. M-CSF is a specific factor in that the proliferation inducing activity is more or less restricted to the macrophage lineage. M-CSF is a potent stimulator of functional activities of monocytes.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family Other Growth Factors/Cytokines
Aliases Macrophage colony-stimulating factor 1, CSF-1, M-CSF, MCSF, Lanimostim
Recombinant Human Macrophage Colony Stimulating Factor (CSF1)
Gene Symbol CSF1
Gene ID 1435
Accession Number P09603
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 18.5 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl (pH 7.5)
Function The ED50 as determined by the dose-dependent stimulation of the proliferation of M-NFS-60 cells was found to be < 1.0 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100655
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg