Recombinant Human Midkine (NEGF2) (E. coli)

Cat. No.
Z102175
Unit
Price
$100.00
Cat. No. Z102175
Name Recombinant Human Midkine (NEGF2) (E. coli)
Description Midkine and its functionally-related protein Pleiotrophin (PTN), are heparin-binding neurotrophic factors that signal through the same receptor. Midkine is involved in a number of biological processes including the migration of neutrophils, inflammatory leukocytes, macrophages, neuronal cells, and osteoclasts. Midkine is a basic, non-glycosylated polypeptide that contains five intra-chain disulfide bonds. There is 87% identity between the human and murine Midkine and approximately 50% identity between human Midkine and human PTN with the conservation of all 10 cysteines.
Organism Human (H. sapiens)
Unit quantity 20 µg
Family VEGF/PGDF/Pleiotrophin Family
Aliases MK, NEGF-2
Gene Symbol MDK
Gene ID 4192
Accession Number P21741
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 14 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered solution in PBS
Function The ED(50) was determined by the dose-dependent proliferation of AsPC-1 cells and was found to be <0.5ng/mL.
Reconstitution Reconstitute at 0.1 mg/ml in sterile PBS
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102175
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