Recombinant Human Nodal

Cat. No.

Z102485

Unit

25 µg

Price

$1,050.00

Cat. No.	Z102485
Name	Recombinant Human Nodal
Description	Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.
Organism	Human (H. sapiens)
Unit quantity	25 µg
Family	TGF-beta Super-family
Tag	Tag Free
Aliases	HTX5
Gene Symbol	NODAL
Gene ID	4838
Accession Number	Q96S42
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	13.1 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
Function	100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells
Reconstitution	Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102485

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.