Recombinant Human Nodal

Cat. No.
Z102485
Unit
25 µg
Price
$850.00
Cat. No. Z102485
Name Recombinant Human Nodal
Description Nodal, also known as Nodal homolog, is a secreted protein member of the Transforming Growth Factor-beta (TGF-beta) superfamily. It is essential for the formation of mesoderm and axial patterning during early vertebrate embryonic development.
Organism Human (H. sapiens)
Unit quantity 25 µg
Family TGF-beta Super-family
Aliases Nodal homolog
Gene Symbol NODAL
Gene ID 4838
Accession Number Q96S42
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 13.1 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
Function 100 ng/mL phosphorylation activity as determined by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells
Reconstitution Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102485
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