Recombinant Human NTF4

Cat. No.

Z100935

Unit

10 µg

Price

$320.00

Cat. No.	Z100935
Name	Recombinant Human NTF4
Description	Neurotrophins are well-known retrograde signaling molecules that regulate differentiation and cell survival in many central and peripheral neurons. Neurotrophin-4 (NT-4) is the most recently discovered neurotrophic factor in mammals and, functionally, the least well understood. Little is known about the role of NT-4 in supporting the survival of identified classes of sensory neurons. Currently, the only available data are from cell counts of whole sensory ganglia. Mice lacking NT-4 (NT-4/) exhibit a loss of ~50% of the neurons in the nodose-petrosal and geniculate ganglia but no apparent loss of neurons in the dorsal root ganglia.
Organism	Human (H. sapiens)
Unit quantity	10 µg
Family	Neuritin/NGF-β/NOTCH Families
Tag	Tag Free
Aliases	GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5
Gene Symbol	NTF4
Gene ID	4909
Accession Number	P34130
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	14 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant NT-4 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.
Function	The ED(50) was determined by the dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures, and was found to be in a range of 10-50 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100935

Print/Download Datasheet

Search CoA here

MSDS

Growth Factors, Cytokines & Kinases MSDS

Other

How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.