Recombinant Human OSM (E. coli)

Cat. No.
Z100755
Unit
Price
$89.00
Cat. No. Z100755
Name Recombinant Human OSM (E. coli)
Description Oncostatin M is produced by monocytes and T cells after cell activation, adherent macrophages, and various T cell lines. Oncostatin M is a member of the IL-6-related cytokine subfamily that includes IL-6, IL-11, LIF, CNTF and CT-1. Oncostatin M mediates its bioactivities through two different heterodimer receptors. They both involve gp130 as a signal transducing moiety, which is found also in receptors for a number of other cytokines. When gp130 dimerizes with LIF receptor beta subunit this generates the high affinity type 1 OSM receptor. When gp130 dimerizes with a protein known as OSM receptor beta this generates the type 2 OSM receptor. Both OSM receptors activate the receptor-associated Janus kinases JAK1, JAK2, and TYK2. Signaling also involves the transcriptional activators STAT3 and STAT5b (see also: STAT proteins) were specifically activated through the gp130-OSM-R-beta type II heterocomplex. The signaling pathway differences observed between the common type I LIF/OSM receptor and the specific type II OSM receptor might explain some of the bioactivities specifically displayed by OSM. Oncostatin M inhibits the growth of several tumor cell lines (A375 melanoma, lung carcinomas). The antiproliferative activity of oncostatin M for some cell lines is synergised by TGF-beta and IFN-gamma. It promotes the growth of human fibroblasts, vascular smooth muscle cells, and some normal cell lines. Oncostatin can inhibit the proliferation of murine M1 myeloid leukemic cells and induces their differentiation into macrophage-like cells, a function shared by LIF, G- CSF, and IL-6.
Organism Human (H. sapiens)
Unit quantity 10 µg
Family MIA(OTOR)/MIF/OSM(LIF) Families
Aliases OSM, Oncostatin M
Gene Symbol OSM
Gene ID 5008
Accession Number P13725
Source E. coli
Species Human (H. sapiens)
Appearance Lyophilized Powder
Molecular Weight 26 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant Oncostatin M was lyophilized from a 0.2μm filtered concentrated (1.0 mg/mL) PBS solution, pH 7.0.
Function The ED(50) was determined by the dose-dependent stimulation of the proliferation of f human TF-1 cells is ≤ 3.0 ng/mL, corresponding to a specific activity of ≥ 2.0 x 10^5 units/mg.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100755
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