Recombinant Human RANTES (CCL5) (E. coli)
| Cat. No. | Z100785 |
| Name | Recombinant Human RANTES (CCL5) (E. coli) |
| Description |
RANTES belongs to the family of chemotactic cytokines known as chemokines and is expressed by an early response gene. The synthesis of RANTES is induced by TNF-alpha and IL-1-alpha. RANTES is chemotactic for T cells, human eosinophils and basophils and plays an active role in recruiting leukocytes into inflammatory sites. RANTES also activates eosinophils to release eosinophilic cationic protein while being a potent activator of oxidative metabolism specific for eosinophils. RANTES increases the adherence of monocytes to endothelial cells and selectively supports the migration of monocytes and T lymphocytes expressing the cell surface markers CD4 and UCHL1. |
| Organism | Human (H. sapiens) |
| Unit quantity | 20 µg |
| Family | Chemokine-beta Family (CC) |
| Aliases |
Regulation upon Activation Normal T cell Express Sequence, CCL5, SIS-delta, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, Small-inducible cytokine A5, T cell-specific protein P228, TCP228, T-cell-specific protein RANTES, D17S136E, SCYA5 |
| Gene Symbol | CCL5 |
| Gene ID | 6352 |
| Accession Number | P13501 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 9 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity |
>95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2µm filtered solution in 50mM NaAc, pH 5.0, 100mM NaCl. |
| Function | Determined by its ability to chemoattract human blood monocytes using a concentration range of 1-10 ng/mL. |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100785 |