Recombinant Human sCD23 (FCER2) (E. coli)

Cat. No.

Z101835

Unit

Price

$253.00

Cat. No.	Z101835
Name	Recombinant Human sCD23 (FCER2) (E. coli)
Description	CD23 is a calcium dependent transmembrane glycoprotein which is expressed in many cell types. The cytoplasmic region of the protein has two isoforms the 'a' isoform that participates in IgE-regulated endocytosis, and the 'b' isoform, which is involved in IgE-regulated phagocytosis. It has been found that there are at least four soluble forms of the CD23 protein (sCD23) all of which are produced by the activity of metallo/cysteine proteases. In addition to binding IgE, sCD23 have been shown to promote the activation and differentiation of B-cells and stimulate the release of pro-inflammatory cytokines from monocytes.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	Other Growth Factors/Cytokines
Tag	Tag Free
Aliases	BLAST-2, CD23, CD23A, CLEC4J, FCE2, FCErII, IGEBF
Gene Symbol	FCER2
Gene ID	2208
Accession Number	P06734
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	19.5 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Function	Measured by its ability to bind in a functional ELISA. Immobilized rhCD23 at 2 Âµg/mL (100 ÂµL/well) can bind human IgE with a linear range of 0.015Â1 Âµg/mL.
Reconstitution	Reconstitute at 100 µg/mL in sterile PBS
Shipping Conditions	This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions.
Storage	Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101835

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.