Recombinant Human TNF (E. coli)
Cat. No.
Z100855
Unit
Price
$92.00
| Cat. No. | Z100855 |
| Name | Recombinant Human TNF (E. coli) |
| Description | Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T cells, natural killer cells following their stimulation by bacterial lipopolysaccharides although cells expressing CD8(+) secrete little or no TNF-alpha. In addition, TNF is secreted by peripheral neutrophilic granulocytes and by a number of transformed cell lines that include those of astrocytes, microglial cells, smooth muscle cells and fibroblasts. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and platelet activating factor. TNF-alpha shares approximately 30% homology with TNF-beta. Human TNF-alpha is a non-glycosylated protein of 17.6 kDa. |
| Organism | Human (H. sapiens) |
| Unit quantity | 50 µg |
| Family | TNF Super-family |
| Tag | Tag Free |
| Aliases | DIF, IMD127, TNF-alpha, TNFA, TNFSF2, TNLG1F |
| Gene Symbol | TNF |
| Gene ID | 7124 |
| Accession Number | P01375 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 17.6 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.2) |
| Function | The ED50 as determined by dose dependent proliferation of IL4 stimulated human megakaryocytic cell line was found to be ≤0.5 ng/mL |
| Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
| Shipping Conditions | This lyophilized protein is shipped at ambient temperature. Cold packs may be used if transit temperatures are elevated. The product remains stable during normal shipping conditions. |
| Storage | Upon receipt, store lyophilized protein at –70°C for long-term storage. Short-term storage at –20°C is acceptable if necessary. Once reconstituted, store at 2–8°C for up to 1 month, or at –20°C for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100855 |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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- Tang, X., McMullen, T. P., & Brindley, D. N. "Increasing the low lipid phosphate phosphatase 1 activity in breast cancer cells decreases transcription by AP-1 and expressions of matrix metalloproteinases and cyclin D1/D3" Theranostics 9(21):6129 (2019).
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