Recombinant Mouse FGF1 (E. coli)

Cat. No.
Z200005
Unit
Price
$89.00
Cat. No. Z200005
Name Recombinant Mouse FGF1 (E. coli)
Description aFGF and bFGF display 55 % homology which is limited, however, to a few functional domains. The structure of aFGF, revealed by X-ray crystallography, shows a similar folding structure as IL-1. Because aFGF binds to the same receptor as bFGF, aFGF displays more or less the same spectrum of activities. It is, however, generally less active than bFGF in similar assays. FGF receptors are encoded by a gene family consisting of at least four receptor tyrosine kinases that transduce signals important in a variety of developmental and physiological processes related to cell growth and differentiation.
Organism Mouse (M. musculus)
Unit quantity 50 µg
Family FGF Family
Aliases aFGF, Fibroblast growth factor 1, FGF-1, Heparin-binding growth factor 1, HBGF-1
Recombinant Mouse Acidic Fibroblast Growth Factor (FGF1)
Gene Symbol FGF1
Gene ID 14164
Accession Number P61148
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 16.0 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant mouse aFGF was lyophilized from a 0.2 μm filtered PBS solution.
Function The ED(50) as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/mL, corresponding to a specific activity of ≥ 1.0 x 10^5 units/mg.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200005
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