Recombinant Mouse FGF8 (E. coli)

Cat. No.
Z200635
Unit
Price
$89.00
Cat. No. Z200635
Name Recombinant Mouse FGF8 (E. coli)
Description FGF-8 (FGF8-b) is a member of the fibroblast growth factor family, which have wide mitogenic and cell survival activities. Additionally, they participate in a number of biological processes during prenatal development, postnatal growth and tissue generation. FGF family members have been implicated in tumour growth, FGF8 specifically in anchorage independent growth of mammary tumour cells. Adult expression of FGF8 is restricted to the testes and ovaries. As an embryonic epithelial growth factor, FGF8 sets up and maintains the midbrain/hindbrain border, and has further roles in limb development, organogenesis and left-axis determination. Recombinant Murine FGF-8b is a 22.5 kDa heparin binding growth factor consisting of 197 amino acids and shares 100% amino acid identity with human FGF-8b.
Organism Mouse (M. musculus)
Unit quantity 50 µg
Family FGF Family
Aliases FGF-8, Androgen-induced growth factor, AIGF, Heparin-binding growth factor 8, HBGF-8 Recombinant Mouse Fibroblast Growth Factor 8 (FGF8)
Gene Symbol FGF8
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 22.5 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 μm filtered solution in 20mM phosphate buffer, 0.2 mM EDTA and 1M NaCl (pH 8.0)
Function The bioactivity was determined in a NIH/3T3 cell proliferation assay. The ED50 was in the range of 0.05 - 0.5 ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favourable performance, avoid repeated handling and multiple freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200635
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