Recombinant Mouse FLT3 Ligand (FLT3LG)

Cat. No.
Cat. No. Z200055
Name Recombinant Mouse FLT3 Ligand (FLT3LG)
Description FLT-3 ligand promotes long-term expansion and differentiation of human pro-B cells in the presence of IL-7 or in combination of IL-7 and IL-3. The human flt-3 ligand also stimulates the proliferation of cells expressing murine flt-3 receptors.n combination with SCF and IL-3 flt-3 ligand can cause expansion of cells with the marker spectrum CD34(+) CD38(-). Alone, flt-3 ligand supports the survival of precursors in the lineage of blood-forming cells such as CFU-GM, CFU-GEMM, and the very primitive high proliferative potential colony-forming cells, HPP-CFC. flt-3 ligand only has marginal effects on progenitors for erythroid cells and megakaryocytes.
Organism Mouse (M. musculus)
Unit quantity 10 µg
Family Other Growth Factors/Cytokines
Aliases FLT-3, Flt3 ligand, Flt3L, SL cytokine,
Gene Symbol FLT3LG
Gene ID 14256
Accession Number P49772
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 19 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant FLT-3 Ligand was lyophilized from a 0.2 μm filtered solution with 50mM Tris,100mM NaCl, pH 8.5.
Function The ED(50) was determined by the dose-dependent proliferation of human AML5 cells was found to be in the range of 10.0 ng/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200055
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg