Recombinant Mouse GM-CSF (CSF2) (E. coli)

Cat. No.
Z200075
Unit
Price
$199.00
Cat. No. Z200075
Name Recombinant Mouse GM-CSF (CSF2) (E. coli)
Description GM-CSF is a monomeric cytokine with two glycosylation sites that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. CSF2 is secreted together with other factors by T cells and macrophages following cell activation by antigens or mitogens. The signalling ‘GM-CSF:receptor’ complex is a dodecamer of two hexamers of two alpha, two beta, and two ligand subunits. While non-glycosylated and glycosylated GM-CSF show similar activities in vitro, fully glycosylated GM-CSF is biologically more active in vivo. Recombinant Mouse CSF2 is a non-glycosylated, monomeric protein.
Organism Mouse (M. musculus)
Unit quantity 20 µg
Family Other Growth Factors/Cytokines
Aliases GMCSF, Granulocyte-macrophage colony-stimulating factor, CSF2
Recombinant Mouse Granulocyte Macrophage Colony Stimulating Factor (CSF2)
Gene Symbol CSF2
Gene ID 12981
Accession Number P01587
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 14 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from 0.2 μm filtered solution in Tris, pH 7.5 and NaCl
Function The ED50 as determined by the dose-dependent stimulation BaF3 cells was < 0.1 ng/mL
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200075
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