Recombinant Mouse IGF1 (E. coli)

Cat. No.
Z200625
Unit
20 µg
Price
$63.00
Cat. No. Z200625
Name Recombinant Mouse IGF1 (E. coli)
Description The Insulin Like Growth Factors, or somatomedins, belong to the Insulin gene family, members of which are insulin and relaxin. The IGFs play similar a similar role to insulin with regards to the regulation of glucose uptake and glycogen metabolism. Additionally IGF1 plays an essential role in mediating many of the effects of growth hormone. IGFs are produced first as inactive precursors, and undergo proteolysis to become active. Circulating IGF1 is produced by hepatocytes whereas local IGF1 is produced in a variety of other tissues where it exhibits paracrine effects. Mouse IGF1 shares 94% and 98% amino acid sequence identity with human and rat IGF1 respectively. Recombinant Mouse IGF1 is a globular, 7.9 kDa protein which contains 3 native disulfide bonds.
Organism Mouse (M. musculus)
Unit quantity 20 µg
Family IGF/Insulin Family
Aliases IGF-I, Mechano growth factor, MGF, Somatomedin-C, IBP1, Recombinant Mouse Insulin Like Growth Factor-I (IGF1)
Gene Symbol IGF1
Accession Number P05017
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 7.9 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method
Purity >95% as determined by SDS-PAGE
Formulation Lyophilized from a 0.2 micron filtered solution in Phosphate buffer and NaCl
Function The ED50 of Recombinant Mouse IGF1 as found by dose dependent proliferation of mouse 3T3 cells was found to be <0.1 ng/ml.
Reconstitution A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200625
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