Recombinant Mouse IL1A (E. coli)

Cat. No.
Cat. No. Z200095
Name Recombinant Mouse IL1A (E. coli)
Description Monocytes are the main source of secreted IL-1. They express predominantly IL-1- beta while human keratinocytes express large amounts of IL-1-alpha. Murine macrophages display a transition from IL-1-beta to IL-1-alpha production during maturation of monocytes into inflammatory macrophages. There are two functionally almost equivalent forms of IL-1, IL-1-alpha and IL-1-beta that are encoded by two different genes. IL-1-beta is the predominant form in humans while it is IL-1-alpha in mice. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL-1-beta but not the IL-1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL-1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T cells and cells of mesenchymal origin. It binds both types of IL-1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.
Organism Mouse (M. musculus)
Unit quantity 10 µg
Family IL-1 Family
Aliases IL-1 alpha, IL1a
Recombinant Mouse Interleukin-1 Alpha (IL1A)
Gene Symbol IL1A
Gene ID 16175
Accession Number P01582
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 18 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant mouse Interleukin-1 alpha was lyophilized from a 0.2 μm filtered PBS solution.
Function The ED(50) was determined by a cell proliferation assay using the murine helper T cell line, D10.G4., was found to be 10.0 pg/mL.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200095
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Controls and Related Product:

1.0 mg

50 µg

50 µg

10 µg