Recombinant Mouse IL2 (E. coli)

Cat. No.
Z200135
Unit
Price
$89.00
Cat. No. Z200135
Name Recombinant Mouse IL2 (E. coli)
Description IL-2 is produced mainly by T cells expressing the surface antigen CD4 following cell activation by mitogens or allogen. Interleukin-2 (IL-2) has multiple, sometimes opposing, functions during an inflammatory response. It is a potent inducer of T cell proliferation and Th1 and Th2 effector T cell differentiation and provides T cells with a long-lasting competitive advantage resulting in the optimal survival and function of memory cells. In a regulatory role, IL-2 is important for the development, survival, and function of regulatory T cells, it enhances Fas-mediated activation-induced cell death, and it inhibits the development of inflammatory Th17 cells. Thus, in its dual and contrasting functions, IL-2 contributes to both the induction and the termination of inflammatory immune responses.
Organism Mouse (M. musculus)
Unit quantity 20 µg
Family Other Interleukins
Aliases IL-2, T-cell growth factor, TCGF
Recombinant Mouse Interleukin-2 (IL2)
Gene Symbol IL2
Gene ID 16183
Accession Number P04351
Source E. coli
Species Mouse (M. musculus)
Appearance Lyophilized Powder
Molecular Weight 17 kDa
Endotoxin Level <1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity >95% as determined by SDS-PAGE
Formulation Recombinant mouse IL-2 was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.
Function The ED50 as determined by the dose dependent stimulation of murine CTLL-2 cells is < 0.1 ng/mL, corresponding to a specific activity of > 1 x 107 units/mg.
Reconstitution A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage For research use only. Not for diagnostic or therapeutic use.
Material Citation If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200135
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